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Ln 3+ also resembles more alkaline earth metal dications than the respective transition metal counterparts, as the bonding with the former is essentially ionic. Lanthanides and Ca 2+ behave as “hard” acids with high affinity to “hard” bases containing oxygen, rather than “soft” bases comprising nitrogen, phosphorus, and sulfur. Notably, the conserved Glu at the last position of the EF-hand binding loop (Glu-12) binds Ca 2+ in a bidentate fashion via both carboxylate oxygens, whereas the other Asp/Glu residues bind Ca 2+ monodentately, employing one of the carboxylate oxygens ( Figure 1).Įmploying Ln 3+ (this includes La 3+ from now on unless specified) in probing Ca 2+ binding sites, which have limited number of chemical properties to be examined by experimental techniques, stems from the ability of the lanthanides to closely mimic some characteristics of the native metal. The binding site is characterized by a pentagonal bipyramidal geometry. Aspartate/glutamate (Asp/Glu) and asparagine/glutamine (Asn/Gln) side chains as well as backbone carbonyls from the loop ligate the Ca 2+ ion, which often retains a bound water molecule. The canonical EF hand motif, which is highly selective for Ca 2+ over Mg 2+ and other physiological metal cations, consists of a 12-residue calcium-binding loop surrounded by two helices creating a signature helix–loop–helix motif. These proteins contain Ca 2+ binding sites, most of which belong to the so-called EF-hand motif family. The latter (parvalbumin, calmodulin D, calcineurin, recoverin, troponin C, cadherin, and S100P) are involved in a plethora of physiological processes such as muscle contraction, vision, cell cycle regulation, brain cortex and cerebellum modulation, and microtubule organization. Lanthanum (La 3+) and its fellow lanthanides (Ln 3+) have been extensively used to study the structure and biochemical properties of vast number of metalloproteins, the calcium-signaling/buffering proteins in particular. Within the series, the competition between La 3+ and its fellow lanthanides is determined by the balance between two competing effects: electronic (favoring heavier lanthanides) and solvation (generally favoring the lighter lanthanides). Solvent exposure of the binding site also influences the process buried active centers with net charge of −4 or −3 are characterized by higher Ln 3+ over Ca 2+ selectivity, whereas it is the opposite for sites with overall charge of −1. The calculations performed reveal that the major determinant of the Ca 2+/Ln 3+ selectivity in calcium proteins is the net charge of the calcium binding pocket the more negative the charge, the higher the competitiveness of the trivalent Ln 3+ with respect to its Ca 2+ contender. A well-calibrated DFT/PCM protocol is employed in studying the factors that control the metal selectivity in biological systems by modeling typical calcium signaling/buffering binding sites and elucidating the thermodynamic outcome of the competition between the “alien” La 3+/Ln 3+ and “native” Ca 2+, and La 3+ − Ln 3+ within the lanthanide series. The present research offers a systematic investigation of the ability of the entire Ln 3+ series to substitute for Ca 2+ in biological systems. The characteristics of lanthanides within the lanthanide series are similar, but not identical. Lanthanides, the 14 4f-block elements plus Lanthanum, have been extensively used to study the structure and biochemical properties of metalloproteins. Over-microwaved chicken is very unforgiving. Place the chicken in a microwave-safe bowl and cover with cling film pierced with a skewer or the tip of a knife. When microwaving chicken it needs to be covered, with a gap for steam to escape. You can cook fish from frozen in the microwave, but this may double the cooking time. If it's not cooked, microwave it in 30-second or 1-minute bursts until cooked through. White fish is good steamed in parcels of baking paper (en papillote, if you will), while it's best to poach salmon or other fatty fish in the sauce you'll be serving it with.Īlways rest the fish for a few minutes before checking it is cooked, as some varieties, such as salmon, may spit a little. The method you choose for microwaving fish will depend on the fish you're cooking and the dish you're making, but poaching and steaming produce the best results. You can buy clever devices that pierce the yolk of a whole egg to create a 'boiled' egg, but do not try this without the right equipment.įish and chicken can be microwaved in less time than it takes to bake or fry them. Never microwave a whole egg or 'fried' egg without piercing the yolk first or, or it's likely to explode and make a mess of your microwave. Do not overcook or you will have a rubbery omelette. Set aside for a minute when almost cooked. Grease a lipped microwave-safe plate with melted butter, then follow the same method as for scrambled eggs, but change the microwaving time to 45–60 seconds.
So if I'm renaming a file named "" it will look it up on TVDB and find "Star Wars: Rebels" is the name of the show, this is Season 4 and Episode 1, and the Title of the Episode is "Heroes of Mandalore (1)" If you're not familiar with using variables like translates to the title of the episode. Then change the path portion of the format. The simple and straightforward answer to this is load a file, look up the info, and then double click (or right click) any item under New Names on the right hand side and select Edit Format. either put an options button on the window or add instructions on how to actually get to the configuration file. nobody is going to know what to change to make this work. I did laugh when I read it, but it is frustrating that you don't answer the simple questions. There is no place at all to configure this software that is obvious to anybody. I am getting an error of a duplicate destination path. there are no instructions with this software. they will try filebot, see that you have to be some kind of advanced coder to understand this software, then uninstall it. you need to inform people where to go and what to click on to get it to work or they wont use it. the question everbody is going to have is where on God's green Earth is the button or file that we need to adjust to get filebot to actually work? filebot does not work straight out of the box. You really don't understand what this guy is asking? how do we configure filebot for plex? you can say "type this in", "Set this to this", or whatever, but you are not helping anybody. I also dont know how to make it fix folders too, im afraid to let it just change entire directories. i want them output to stay exactly where they are and just fix the names ! i only have 20 GB of space left on my SSD OS drive and i cant rename any moves or tv shows cause there is no space, it moves them to C: !Īll my plex media resides on a drivepool of 16 TB called "PLEX (P:)"Ģ) i would love to know if im using the best scripts and formats for my PLEX drive media. I have used it a couple of times and fixed some things but still not sure how to do it in a systematic step manner so i know exactly what im doing and where.ġ) when i rename files it MOVES them to C drive under users and Plex media folder. What i do know is i have a huge PLEX data base drivepool that im trying to fix up and organize the right way and im OCD about arranging it and people tell me filebot is what i need. I really dont know how to use it exactly i have following instructions but most seem a little fuzzy for me, i have no background in scripting and what not. I am very new to filebot and i am having some issues. The Curved Ruler: For projects other than squared quilts, like round-shaped cutting a perfect curved patch of fabric is required. If the quilting project requires innovative forms like wheels, use this type. The Pyramid Ruler: Also known as the Dresden ruler, have a pyramidal triangular shape with more extended heights but short bases. For getting the perfect right angles and making the work of cutting the exact sizes of fabric, this ruler is a must. For preparing a quilt with perfect squares, use this kind of quilting ruler. The Squared Ruler: They are in the form of a triangular square and half square. Following the marked angles properly will ensure accurately fitting pieces. Cutting fabric pieces become a lot easier with this type of quilting ruler. 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It also likes to be intentionally deceptive when it sets up a section’s rules via tutorial puzzles. But as you traverse more of the island, the line between puzzles that you can’t possibly solve because the rules haven’t been explained to you, and puzzles that are possible to solve but just really hard, begins to blur.Įventually for me, that line vanished, and that’s when the game went from challenging, to frustrating and borderline unfair at times. When this first happens, you quickly figure it out and abandon a confusing section for returning to later. Some areas’ puzzles are built on rules established in other areas, so traveling to them out of order will present you with seemingly hopelessly complex mazes beyond your current comprehension of the game’s mechanics. While you can go damn near anywhere to start, you damn well shouldn’t. This open-endedness is where the game begins to fall apart. Letting you find your way on your own.Īn early puzzle that breaks your brain, but has a clear rule set. They escalate slowly, holding your hand through the first few rule sets before casting all tutorializing aside to let you explore (nearly) all of the island from the get go. The Witness is an absolutely stunning looking game, and the world it places you in is wonderfully tantalizing, with gorgeous yet barren environments hinting at a grander story buried under the island’s quiet facade. Do that several times and…more things happen.Īt the start, things are great. Solve enough puzzles in any given region, and a massive laser cannon shoots up a sculpture found atop a mountain. Many times you’re asked to do both, while sometimes incorporating hints you find in the surroundings to help you solve the puzzle and move onto the next. Others may ask you to form certain shapes with the path you create. Some may have you separate certain shapes while following a certain rule set. The mazes start out simple, but quickly snowball in complexity. Gameplay consists entirely of traversing the island and solving said puzzles, which more often than not take the form of small mazes on panels you interact with. You just wake up in a tunnel and start solving puzzles to make your way through the various environments of the island, which range from mysterious desert ruins, abandoned buildings and giant castles. Hints of an amazing lost world fill the otherwise sterile environments.įor those who may not know, The Witness is a first-person adventure game where you play as an unnamed protagonist on a mysterious island who tasked to solve puzzles for some reason or another. That being said, even I was surprised by the gulf between the critical consensus of and my personal views on The Witness, a game that many critics are lauding as one of the greatest of all time, and a game that I feel is a worthless exploration into just how little a game developer can care about his audience. I hate a lot of popular games, but I realize that in many cases the issue is with me, not the game. My point is that I am more than used to not being in jive with the critical consensus, and with rare exception I caulk it up to being an outlier. Dark Souls, Metal Gear (any of them), Undertale, The Witcher, The Walking Dead, I either hated them or couldn’t even be bothered to start them because they looked so boring, derivative or just not something I’d be into. The Batman Arkham games? Pretty vacant, with repetitive (and unresponsive) combat coupled with the pathetically heavy-handed writing. The original Dragon Age? 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